False-Positive T-Cell Cytotoxicity Crossmatch Results Due to Autoantibodies in Korean Network for Organ Sharing Crossmatch Tests

Positive virtual crossmatch with negative flow crossmatch results in two cases.

Pre-transplant (Tx) presence of HLA antibodies (HLA-Ab) especially donor specific antibodies (DSA) has been correlated with post-Tx rejection. While crossmatch (XM) is the specific method to identify DSA, logistical reasons prevent performing a prospective XM in all transplants. In such cases DSA as identified by solid-phase assay (SPA) are being used to perform a virtual crossmatch (VXM). We p...

Living-donor kidney transplant in T-cell and B-cell flow cytometry crossmatch-positive patients.

OBJECTIVES Complement-dependent cytotoxic crossmatch is an important indicator for kidney transplant. However, there is controversy about treatment for flow cytometry crossmatch-positive cases. MATERIALS AND METHODS This was a retrospective study of 127 living-donor kidney transplant recipients from May 2007 to July 2011. We divided patients into 115 flow cytometry crossmatch T-cell and B-cel...

Successful liver transplantation from crossmatch-positive donors.

The impact of positive T-cell lymphocytotoxic crossmatch on intestinal allograft rejection and survival.

Over a 9-year period, 124 consecutive patients received a total of 130 intestinal allografts at our center: 51 isolated intestines and 79 composite visceral grafts (62 liver/intestine and 17 multivisceral). Of these, 51 % were male and 58% were children. All donors were cadaveric and ABO identical. The HLA match was random and no attempts were made to immunomodulate the graft. The baseline immu...

False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor

Crossmatching is essential prior to kidney transplantation to confirm compatibility between the donor and the recipient, particularly to prevent acute antibody-mediated rejection. An unexpected positive crossmatch may be obtained in recipients with an autoimmune disease or preexisting antibodies not detected by single-antigen bead array due to complement interference or who have been previously...